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elements that carry them (Box 8.10) makes it clear that a combination of gene duplication, mutational re nement, recombination, and proliferation was the broad mechanism that made atrazine biodegradable around the globe today. The last case study in Box 8.10 describes microbial metabolism of chlorobenzene. The scenario portrayed for chlorobenzene is very different from that of atrazine because it is site-speci c. Chlorobenzene is a colorless organic liquid with a faint, almond-like odor whose primary industrial use is the manufacture of dyestuffs and insecticides. It is also a solvent for adhesives, drugs, rubber, paints, dry cleaning, and textile manufacture. Long-term exposure to chlorobenzene may damage the liver, kidney, and central nervous system of humans. Information presented in Box 8.10 describes how chlorobenzene spilled into the subsurface at Kelly Air Force Base (near San Antonio, Texas), persisted for several decades, and then the concentrations began to decline. Culturable bacteria capable of growing on two compounds related to chlorobenzene (toluene, chlorocatechol) were found throughout the study site. Only after natural attenuation (see Section 8.3) of chlorobenzene had begun, was it possible to isolate (from the contaminated portion of the study site) bacteria that utilized chlorobenzene as a carbon and energy source. Biochemical and genetic analysis of the genes conferring chlorobenzene metabolism on the newly isolated bacterium revealed that the new gene cluster was a hybrid of those from toluene and chlorocatechol metabolic pathways and that two mobile genetic elements (a genomic island and transposons) were the likely causes of site-speci c evolution of chlorobenzene catabolism (Box 8.10). barcode formula for excel 2007 Barcode Excel Add-In TBarCode Office: Create Barcodes in Excel
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